Molecular biology of nickel carcinogenesis: Identification of differentially expressed genes in morphologically transformed C3H10T1/2 Cl 8 mouse embryo fibroblast cell lines induced by pecific insoluble nickel compounds
Identifieur interne : 003137 ( Main/Exploration ); précédent : 003136; suivant : 003138Molecular biology of nickel carcinogenesis: Identification of differentially expressed genes in morphologically transformed C3H10T1/2 Cl 8 mouse embryo fibroblast cell lines induced by pecific insoluble nickel compounds
Auteurs : Rini Verma [États-Unis] ; Jamuna Ramnath [États-Unis] ; Farrah Clemens [États-Unis] ; Lisa C. Kaspin [États-Unis] ; Joseph R. Landolph [États-Unis]Source :
- Molecular and Cellular Biochemistry [ 0300-8177 ] ; 2004-01-01.
English descriptors
- KwdEn :
Abstract
Abstract: Inhalation of mixtures of insoluble and soluble nickel compounds by humans during nickel refining has been associated with excess lung and nasal sinus cancers. Insoluble nickel subsulfide (Ni3S2) and nickel oxide (NiO) are carcinogenic to rodents by inhalation. We previously showed that insoluble Ni3S2, crystalline nickel monosulfide (NiS), and green (high temperature, HT) and black (low temperature, LT) NiO, induced morphological transformation in cultured C3H/10T1/2 Cl 8 (10T1/2) mouse embryo cells. To understand molecular mechanisms of carcinogenesis by insoluble nickel compounds, we used random, arbitrarily primed-polymerase chain reaction (RAP-PCR) mRNA differential display and identified nine cDNA fragments that were differentially expressed between nontransformed and nickel-transformed cell lines in ~ 10.0% of the total mRNA. Expression of the calnexin gene (encoding a type I membrane protein/molecular chaperone), the ect-2 proto-oncogene, and the stress-inducible gene, Wdr1, was upregulated. Expression of six genes – the vitamin D interacting protein/thyroid hormone activating protein 80 (DRIP/TRAP-80) gene, the insulin-like growth factor receptor 1 (IGFR1) gene, the small nuclear activating protein (SNAP C3) gene, and three unknown genes, was down-regulated, in nickel-transformed cell lines. We hypothesize that these resulting aberrations in gene expression could contribute to the induction and/or maintenance of morphological transformation induced by specific insoluble nickel compounds.
Url:
DOI: 10.1023/B:MCBI.0000007276.94488.3d
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002324
- to stream Istex, to step Curation: 002324
- to stream Istex, to step Checkpoint: 000B95
- to stream Main, to step Merge: 003169
- to stream Main, to step Curation: 003137
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular biology of nickel carcinogenesis: Identification of differentially expressed genes in morphologically transformed C3H10T1/2 Cl 8 mouse embryo fibroblast cell lines induced by pecific insoluble nickel compounds</title><author><name sortKey="Verma, Rini" sort="Verma, Rini" uniqKey="Verma R" first="Rini" last="Verma">Rini Verma</name></author><author><name sortKey="Ramnath, Jamuna" sort="Ramnath, Jamuna" uniqKey="Ramnath J" first="Jamuna" last="Ramnath">Jamuna Ramnath</name></author><author><name sortKey="Clemens, Farrah" sort="Clemens, Farrah" uniqKey="Clemens F" first="Farrah" last="Clemens">Farrah Clemens</name></author><author><name sortKey="Kaspin, Lisa C" sort="Kaspin, Lisa C" uniqKey="Kaspin L" first="Lisa C." last="Kaspin">Lisa C. Kaspin</name></author><author><name sortKey="Landolph, Joseph R" sort="Landolph, Joseph R" uniqKey="Landolph J" first="Joseph R." last="Landolph">Joseph R. Landolph</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:839648DB94EAE683AAC34DFCFDBC6E1772CBC9B1</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1023/B:MCBI.0000007276.94488.3d</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-38H4QV50-T/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002324</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002324</idno><idno type="wicri:Area/Istex/Curation">002324</idno><idno type="wicri:Area/Istex/Checkpoint">000B95</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B95</idno><idno type="wicri:doubleKey">0300-8177:2004:Verma R:molecular:biology:of</idno><idno type="wicri:Area/Main/Merge">003169</idno><idno type="wicri:Area/Main/Curation">003137</idno><idno type="wicri:Area/Main/Exploration">003137</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Molecular biology of nickel carcinogenesis: Identification of differentially expressed genes in morphologically transformed C3H10T1/2 Cl 8 mouse embryo fibroblast cell lines induced by pecific insoluble nickel compounds</title><author><name sortKey="Verma, Rini" sort="Verma, Rini" uniqKey="Verma R" first="Rini" last="Verma">Rini Verma</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology and USC/Norris Comprehensive Cancer Center Keck School of Medicine, University of Southern California, 90089, Los Angeles, CA</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Ramnath, Jamuna" sort="Ramnath, Jamuna" uniqKey="Ramnath J" first="Jamuna" last="Ramnath">Jamuna Ramnath</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology and USC/Norris Comprehensive Cancer Center Keck School of Medicine, University of Southern California, 90089, Los Angeles, CA</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Clemens, Farrah" sort="Clemens, Farrah" uniqKey="Clemens F" first="Farrah" last="Clemens">Farrah Clemens</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology and USC/Norris Comprehensive Cancer Center Keck School of Medicine, University of Southern California, 90089, Los Angeles, CA</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kaspin, Lisa C" sort="Kaspin, Lisa C" uniqKey="Kaspin L" first="Lisa C." last="Kaspin">Lisa C. Kaspin</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology and USC/Norris Comprehensive Cancer Center Keck School of Medicine, University of Southern California, 90089, Los Angeles, CA</wicri:regionArea><orgName type="university">Université de Californie du Sud</orgName><placeName><settlement type="city">Los Angeles</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Landolph, Joseph R" sort="Landolph, Joseph R" uniqKey="Landolph J" first="Joseph R." last="Landolph">Joseph R. Landolph</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology, Department of Pathology, USC/Norris Comprehensive Cancer Center Keck School of Medicine</wicri:regionArea><wicri:noRegion>USC/Norris Comprehensive Cancer Center Keck School of Medicine</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and Cellular Biochemistry</title><title level="j" type="sub">An International Journal for Chemical Biology in Health and Disease</title><title level="j" type="abbrev">Mol Cell Biochem</title><idno type="ISSN">0300-8177</idno><idno type="eISSN">1573-4919</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2004-01-01">2004-01-01</date><biblScope unit="volume">255</biblScope><biblScope unit="issue">1-2</biblScope><biblScope unit="page" from="203">203</biblScope><biblScope unit="page" to="216">216</biblScope></imprint><idno type="ISSN">0300-8177</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8177</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>C3H/10T1/2 mouse embryo cells</term><term>RAP-PCR differential display</term><term>Wdr1</term><term>calnexin</term><term>carcinogenic insoluble nickel compounds</term><term>chemical carcinogens</term><term>ect-2</term><term>morphological transformation</term><term>thyroid hormone receptor activating protein (TRAP)</term><term>vitamin D receptor interacting protein (DRIP)</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Inhalation of mixtures of insoluble and soluble nickel compounds by humans during nickel refining has been associated with excess lung and nasal sinus cancers. Insoluble nickel subsulfide (Ni3S2) and nickel oxide (NiO) are carcinogenic to rodents by inhalation. We previously showed that insoluble Ni3S2, crystalline nickel monosulfide (NiS), and green (high temperature, HT) and black (low temperature, LT) NiO, induced morphological transformation in cultured C3H/10T1/2 Cl 8 (10T1/2) mouse embryo cells. To understand molecular mechanisms of carcinogenesis by insoluble nickel compounds, we used random, arbitrarily primed-polymerase chain reaction (RAP-PCR) mRNA differential display and identified nine cDNA fragments that were differentially expressed between nontransformed and nickel-transformed cell lines in ~ 10.0% of the total mRNA. Expression of the calnexin gene (encoding a type I membrane protein/molecular chaperone), the ect-2 proto-oncogene, and the stress-inducible gene, Wdr1, was upregulated. Expression of six genes – the vitamin D interacting protein/thyroid hormone activating protein 80 (DRIP/TRAP-80) gene, the insulin-like growth factor receptor 1 (IGFR1) gene, the small nuclear activating protein (SNAP C3) gene, and three unknown genes, was down-regulated, in nickel-transformed cell lines. We hypothesize that these resulting aberrations in gene expression could contribute to the induction and/or maintenance of morphological transformation induced by specific insoluble nickel compounds.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>Los Angeles</li></settlement><orgName><li>Université de Californie du Sud</li></orgName></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Verma, Rini" sort="Verma, Rini" uniqKey="Verma R" first="Rini" last="Verma">Rini Verma</name></region><name sortKey="Clemens, Farrah" sort="Clemens, Farrah" uniqKey="Clemens F" first="Farrah" last="Clemens">Farrah Clemens</name><name sortKey="Kaspin, Lisa C" sort="Kaspin, Lisa C" uniqKey="Kaspin L" first="Lisa C." last="Kaspin">Lisa C. Kaspin</name><name sortKey="Landolph, Joseph R" sort="Landolph, Joseph R" uniqKey="Landolph J" first="Joseph R." last="Landolph">Joseph R. Landolph</name><name sortKey="Landolph, Joseph R" sort="Landolph, Joseph R" uniqKey="Landolph J" first="Joseph R." last="Landolph">Joseph R. Landolph</name><name sortKey="Ramnath, Jamuna" sort="Ramnath, Jamuna" uniqKey="Ramnath J" first="Jamuna" last="Ramnath">Jamuna Ramnath</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003137 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003137 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:839648DB94EAE683AAC34DFCFDBC6E1772CBC9B1
|texte= Molecular biology of nickel carcinogenesis: Identification of differentially expressed genes in morphologically transformed C3H10T1/2 Cl 8 mouse embryo fibroblast cell lines induced by pecific insoluble nickel compounds
}}
| This area was generated with Dilib version V0.6.33. |  |